Mechanical Sensitivity Assessment in Sciatic Nerve Injury

In examining sciatic nerve sensory functionality, the threshold of paw withdrawal was measured in response to mechanical stimulation with the aid of von Frey filament (Ugo Basile, Italy); 10 filaments were selected.[20 (link)] The rats were kept in plastic cages with a metal mesh basement and were allowed to be accustomed to the environment for 10 min before testing. von Frey filaments with the order of forces, 4.17 g, 4.31 g, 4.56 g, 4.74 g, 4.93 g, 5.07 g, 5.18 g, 5.46 g, 5.88 g, and 6.1 g, were applied to the plantar surface of the hind paw. Each paw was tested 5 times, mechanical pain threshold was estimated according to the up and down method used by Bonin et al.[21 (link)]
Pre-induction (Pre-I or BL) nociceptive threshold were assessed (2 days before CCI) with von Frey filament to determine the basal threshold, post-induction (Post-I) nociceptive threshold were assessed 3 days after CCI intervention and then on the 10^th, 17^th, and 24^th day of the commencement of drug treatments.