Tamoxifen-Induced Gene Deletion in Mice

Wild-type (WT) C57BL/6 (B6) mice were purchased from Koatech. Raptor^fl/fl, Rictor^fl/fl, and Pten^fl/fl mice [44 (link),45 (link)] were crossed with the tamoxifen-inducible Cre (Ert2Cre) transgenic mice (B6.129-Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj/J}, The Jackson Laboratory). To delete floxed genes, tamoxifen (0.1 mg/ear, Sigma-Aldrich, St. Louis, MO, USA) was applied to the ear skin daily for 5 days. Genotyping was performed by using PCR or immunoblotting. All animal experimentations were conducted in accordance with guidelines and approval of the International Animal Care and Use Committees of Hallym University (Hallym 2020-28, Hallym 2021-59).

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Gupta A., Lee K, & Oh K. (2023). mTORC1 Deficiency Prevents the Development of MC903-Induced Atopic Dermatitis through the Downregulation of Type 2 Inflammation. International Journal of Molecular Sciences, 24(6), 5968.

Publication 2023

C57BL/6 (B6) mice tamoxifen Ert2Cre) B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J,

Animal Ert2 Genes Mice Rosa Skin Tamoxifen Transgenic mice

Corresponding Organization : Hallym University

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Variable analysis

independent variables

Genotype: Raptor^(fl/fl), Rictor^(fl/fl), and Pten^(fl/fl) mice crossed with tamoxifen-inducible Cre (Ert2Cre) transgenic mice
Tamoxifen treatment: Daily application of tamoxifen (0.1 mg/ear) for 5 days to delete floxed genes

dependent variables

Not explicitly mentioned

control variables

Wild-type (WT) C57BL/6 (B6) mice
Genotyping: PCR or immunoblotting

controls

Positive control: Tamoxifen-inducible Cre (Ert2Cre) transgenic mice
Negative control: Wild-type (WT) C57BL/6 (B6) mice

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