Electrochemical Probe Fabrication Protocol

Wild-type streptavidin tetramer was purchased from Sigma-Aldrich. The biotin disulfides were synthesized according to a previous report (22 (link)) and treated by the immobilized (tris[2-carboxyethyl]phosphine hydrochloride) disulfide reducing gel from Thermo Fisher Scientific (catalog number 77712) following the manufacturer’s instructions. All other chemicals used in the probe fabrication and electrochemical characterization were bought from Sigma-Aldrich, apart from the plating solution. The plating solution was obtained from Tianyue (China), which has 5 g in 250 ml of electrolyte.

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Tang L., Yi L., Jiang T., Ren R., Paulose Nadappuram B., Zhang B., Wu J., Liu X., Lindsay S., Edel J.B, & Ivanov A.P. (2022). Measuring conductance switching in single proteins using quantum tunneling. Science Advances, 8(20), eabm8149.

Publication 2022

(tris[2-carboxyethyl]phosphine hydrochloride) disulfide reducing gel

Biotin Disulfide Electrolyte Phosphine Streptavidin Tetramer Tris

Corresponding Organization : Imperial College London

Other organizations : University of Strathclyde, Arizona State University

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Variable analysis

independent variables

Concentration of biotin disulfides

dependent variables

Electrochemical characterization of the probe

control variables

Wild-type streptavidin tetramer
Biotin disulfides synthesized according to a previous report
Immobilized (tris[2-carboxyethyl]phosphine hydrochloride) disulfide reducing gel from Thermo Fisher Scientific
Plating solution obtained from Tianyue (China)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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