Metastatic Pancreatic Cancer Cell Lines and PDXs

The BxPC3, Sw1990, AsPC-1, and CFPAC pancreatic cancer cell lines and the SKBR3 breast cancer cell line were from ATCC (Rockville, MD) and were cultured following the ATCC recommendations. The two PDXs (P4604 and P2846) were generated from resected hepatic and peritoneum metastatic samples, respectively, from two patients with pancreatic cancer treated with gemcitabine (P4604) or untreated at surgery time (P2846) (PDX Platform, Institut de Recherche en Cancérologie de Montpellier) (58 (link)). The PDX cell line C-PDX P4604 was derived from the PDX P4604. The gemcitabine-resistant cell line BxPC3-GR was generated by exposure to progressively increasing doses of gemcitabine, as previously described (58 (link)). All cell lines and the PDXs express EGFR at high level (between 150,000 and 350,000 receptors/cell), HER2 at intermediate level (between 6,000 and 78,000 receptors/cell), and HER3 at low level (between 5,000 and 20,000 receptors/cell) (26 (link), 58 (link), 59 (link)), except the SKBR3 cell line that is HER2^high (>1 million receptor/cell) (59 (link)). The CD16/FcγRIII (158V allotype)-transfected human NK92 cell line was cultured in complete RPMI medium supplemented with 100 IU/ml interleukin 2, as previously described (60 (link)).