Competitive Dye Displacement Assay for CMT-AgNPs–ctDNA Binding

Horiba-Fluoromax Spectrofluorometer is employed for all fluorescence measurements. The competitive dye displacement assay is done to unravel the binding mode of the CMT-AgNPs–ctDNA complex^{31 (link)}. The DNA binding dyes Hst and MetG were used for the assay as it has an intense fluorescence intensity due to their known minor and major groove mode of binding with DNA respectively^{32 (link),33 (link)}. Thus, the nature of interaction or binding could be easily identified by tracking the modifications in fluorescence intensities of the dye–DNA complex by adding CMT-AgNPs.

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Raj A., Thomas R.K., Vidya L., Aparna V.M., Neelima S, & Sudarsanakumar C. (2023). Exploring the cytotoxicity on human lung cancer cells and DNA binding stratagem of camptothecin functionalised silver nanoparticles through multi-spectroscopic, and calorimetric approach. Scientific Reports, 13, 9045.

Publication 2023

Fluoromax Spectrofluorometer

Assay Dyes Fluorescence Fluorescence dye

Corresponding Organization :

Other organizations : Mahatma Gandhi University

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Variable analysis

independent variables

Concentration of CMT-AgNPs

dependent variables

Fluorescence intensity of Hst-DNA complex
Fluorescence intensity of MetG-DNA complex

control variables

Concentration of Hst and MetG dyes
Concentration of ctDNA

controls

Positive control: Hst-DNA complex and MetG-DNA complex without CMT-AgNPs
Negative control: Not specified

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