Immunomodulation of PMN-MDSCs in 4T1 Breast Tumors
PMN-MDSCs were isolated from Balb/C mice injected with 4T1 breast tumor cells and treated with NaCl (Ctrl) +/− doxorubicin +/− GTN. At day 23, the tumors were recovered and PMN-MDSCs were isolated as previously described [17 (link)]. PMN-MDSCs were cultured alone or co-cultured for 5 days with T lymphocytes activated by anti-CD3/anti-CD28 beads and labeled with cell traceTM violet (Invitrogen, Waltham, MA, USA). T lymphocytes were previously isolated from spleens and lymph nodes of naive mice using the Pan T cells isolation kit II (Miltenyi, Bergisch Gladbach, Germany).
Mabrouk N., Racoeur C., Shan J., Massot A., Ghione S., Privat M., Dondaine L., Ballot E., Truntzer C., Boidot R., Hermetet F., Derangère V., Bruchard M., Végran F., Chouchane L., Ghiringhelli F., Bettaieb A, & Paul C. (2023). GTN Enhances Antitumor Effects of Doxorubicin in TNBC by Targeting the Immunosuppressive Activity of PMN-MDSC. Cancers, 15(12), 3129.
Anti cd3 Balb c mice Breast tumor Cell Cells isolationDoxorubicin Lymph nodes Mdscs Mice Nacl T lymphocytes Tumors Violet
Corresponding Organization :
Other organizations :
École Pratique des Hautes Études, Université de Bourgogne, Université Paris Sciences et Lettres, Weill Cornell Medical College in Qatar, Centre Georges François Leclerc, Centre National de la Recherche Scientifique, Inserm
Proliferation of T lymphocytes co-cultured with PMN-MDSCs
control variables
Balb/C mice
4T1 breast tumor cells
PMN-MDSCs isolated from tumor-bearing mice
T lymphocytes isolated from naive mice
T lymphocytes activated by anti-CD3/anti-CD28 beads
T lymphocytes labeled with cell traceTM violet
controls
Negative control: PMN-MDSCs cultured alone
Positive control: T lymphocytes activated by anti-CD3/anti-CD28 beads
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