Authenticating Cell Lines and PDX Models

Cell lines and PDX models were routinely authenticated by SNP fingerprinting and tested for mycoplasma. HPB-ALL was obtained from Andreas Strasser. TALL-1, RPMI-8402, SUPT11, Loucy, P12-Ichikawa, PF382 were purchased from DSMZ. Jurkat (clone E6-1), U2OS, MDA-MB-468, HCC1143 were purchased from ATCC. HLR PathDetect cells were purchased from Stratagene. All other cell lines were obtained from the Novartis stocks of the Cancer cell line encyclopedia (27 (link)). T-ALL PDX models were created from primary T-ALLs by injection by tail vein into immunodeficient NOD/Scid/IL2rγ^null (NSG) mice and are described at the online portal PRoXe at http://PRoXe.org. Detergent extracts of T-ALL cells obtained from the marrows of leukemia-bearing mice were analyzed for activated NOTCH1 (ICD1) and activated NOTCH3 (ICD3) by Western blotting.

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Bernasconi-Elias P., Hu T., Jenkins D., Firestone B., Gans S., Kurth E., Capodieci P., Deplazes-Lauber J., Petropoulos K., Thiel P., Ponsel D., Choi S.H., LeMotte P., London A., Goetcshkes M., Nolin E., Jones M.D., Slocum K., Kluk M.J., Weinstock D.M., Christodoulou A., Weinberg O., Jaehrling J., Ettenberg S.A., Buckler A., Blacklow S.C., Aster J.C, & Fryer C.J. (2016). Characterization of activating mutations of NOTCH3 in T cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies. Oncogene, 35(47), 6077-6086.

Publication 2016

TALL-1 RPMI-8402 SUPT11 Loucy P12-Ichikawa PF382 Jurkat (clone E6-1), MDA-MB-468 HCC1143

Cancer Cell line Cells Cells extracts Clone Detergent Immunodeficient Leukemia Marrows Mice Mycoplasma Notch3 Scid T all Tail Tall 1 Vein

Corresponding Organization :

Other organizations : Pathways Behavioral Services, MorphoSys (Germany), Dana-Farber Cancer Institute, Brigham and Women's Hospital, Boston Children's Hospital, Harvard University

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Variable analysis

independent variables

Cell lines and PDX models obtained from different sources (e.g., DSMZ, ATCC, Novartis Cancer cell line encyclopedia)

dependent variables

Activated NOTCH1 (ICD1) and activated NOTCH3 (ICD3) levels in T-ALL cells obtained from the marrows of leukemia-bearing mice, as measured by Western blotting

control variables

Cell line authentication by SNP fingerprinting
Testing for mycoplasma contamination
Use of immunodeficient NOD/Scid/IL2rγnull (NSG) mice for T-ALL PDX model generation

positive controls

None specified

negative controls

None specified

Annotations

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