Protein Expression Analysis via Western Blot

The protein expression of p-JAK1, 2, 3 (Rabbit mAb; CST), p-STAT1, 2, 3 (Rabbit mAb; CST), CDK2 (Rabbit Polyclonal; Proteintech), cyclin E (Rabbit Polyclonal; Proteintech), p21 (Rabbit Polyclonal; Proteintech), VPS25 (Rabbit Polyclonal; Proteintech), METTL3, METTL14 and YTHDC1 (Rabbit Polyclonal; Proteintech) was detected by western blot as described previously [23 (link), 24 (link)]. Briefly, cells were lysed by Sample Buffer, Laemmli 2 × Concentrate (Sigma, MO, USA) via the manufacturer's protocol. Next, the proteins were isolated by 10% SDS–PAGE gel and transferred to nitrocellulose membranes. After immunoblotting with primary and secondary antibodies, the protein bands were incubated with enhanced chemiluminescence (ECL) and visualized using a Tanon 5200 Chemiluminescence Imaging System (Shanghai, China).

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Zhu X., Yang H., Zhang M., Wu X., Jiang L., Liu X, & Lv K. (2021). YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells. Cancer Cell International, 21, 645.

Publication 2021

cyclin E METTL3 METTL14 YTHDC1

Antibodies Buffer Cdk2 Cells Chemiluminescence Cyclin e Jak1 Membranes Mettl14 Mettl3 Nitrocellulose Protein Rabbit Sds page Stat1 Western blot

Corresponding Organization :

Other organizations : First Affiliated Hospital of Wannan Medical College, Wannan Medical College

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Variable analysis

independent variables

P-JAK1, 2, 3 (Rabbit mAb; CST)
P-STAT1, 2, 3 (Rabbit mAb; CST)
CDK2 (Rabbit Polyclonal; Proteintech)
Cyclin E (Rabbit Polyclonal; Proteintech)
P21 (Rabbit Polyclonal; Proteintech)
VPS25 (Rabbit Polyclonal; Proteintech)
METTL3, METTL14 and YTHDC1 (Rabbit Polyclonal; Proteintech)

dependent variables

Protein expression levels

control variables

Cells were lysed by Sample Buffer, Laemmli 2 × Concentrate (Sigma, MO, USA) via the manufacturer's protocol
Proteins were isolated by 10% SDS–PAGE gel and transferred to nitrocellulose membranes
After immunoblotting with primary and secondary antibodies, the protein bands were incubated with enhanced chemiluminescence (ECL) and visualized using a Tanon 5200 Chemiluminescence Imaging System (Shanghai, China)

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