Eighty milliliters of the water enriched with microorganisms used for the biodegradation tests was filtered (0.2μm porosity, cellulose nitrate filters, Sartorius Stedim). The filters were then ground in liquid nitrogen to a fine powder, and DNA was extracted using the Mobio-Power Soil DNA isolation kit (Mobio Laboratories, Carlsbad, CA) in line with the supplier’s recommendations. The genomic DNA was sent to a commercial company (MR DNA, Shallowater, Texas, United States). Following the amplification of the V4 hypervariable region of the 16S rRNA gene (PCR primers 515/806), the amplicons were sequenced via MiSeq 2 × 300bp sequencing (Illumina, California, United States) in line with the supplier’s recommendations. The sequences were converted and demultiplexed with QIIME1. The sequences were analyzed using the software package DADA2 (Callahan et al., 2016 (link)) in the QIIME2 pipeline. With this package, the sequences were treated by quality filtering, merging, dereplicating, and removing chimeras to determine amplicon sequence variants (ASVs). Taxonomy was assigned using VSearch (Rognes et al., 2016 (link)) against the SILVA SSU 138 NR database (Quast et al., 2013 (link)) without uncultured/environmental sequences. The raw data were deposited in the NCBI SRA under bioproject ID PRJNA739070.
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