KPC/vector and KPYC/Flag-Yap lines were established previously and cultured on collagen-coated plates in Waymouth MB 752/1 Medium (Sigma-Aldrich, St. Louis, MO) containing 10% FBS (Zhang et al 2014 (link)). KPC cells established from a KPC PDAC tumor were stably transduced with pTRIPZ or pTRIPZ-Yapsh and maintained in DMEM medium (Corning, Corning, NY) containing 10% fetal bovine serum (FBS). Yapsh expression was induced by treating the cells with Doxcycline (4 µg/mL) for 3 days. Regularly mycoplasma testing was performed in house using Mycoplasma Detection Kit (InvivoGen, San Diego, CA).
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Establishment and Maintenance of KPC and KPYC Cell Lines
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Corresponding Organization :
Other organizations : Georgetown University, Georgetown University Medical Center
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Variable analysis
independent variables
- Yapsh expression
- Doxycycline treatment
- Cell proliferation
- Cell migration
- Cell invasion
- Tumor growth
- Collagen-coated plates
- Waymouth MB 752/1 Medium (Sigma-Aldrich, St. Louis, MO) containing 10% FBS
- DMEM medium (Corning, Corning, NY) containing 10% fetal bovine serum (FBS)
- Mycoplasma testing
- Positive control: KPC/vector and KPYC/Flag-Yap lines
- Negative control: Not explicitly mentioned
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