Tea Transcriptome PMEI Protein Identification

Before the tea plant genome sequenced, 20 expressed sequence tags (EST) that annotated as PMEI domain containing proteins were identified from the transcriptome data of the tea plant under CA condition (Wang et al., 2013 (link)). After assembled by Seqman software, a total of 4 contigs were obtained and served as templates to design RT-PCR primers for TA cloning. The TA cloning method was performed as described by Qian et al. (2016) (link). The amplified and purified PCR products were inserted into the pEASY-Blunt Zero vector (TransGen Biotech, Beijing, China), then transferred into Trans5α chemically competent cell (TransGen Biotech, Beijing, China) and sequenced finally. All RT-PCR primers were listed in Supplementary Table 3.