Epileptic Activity Scoring in Mice

Epileptic activity of mice induced by tutin was scored for 2 h using a modified Racine scale as follows^{48 (link)}: Stage 0: no reaction; Stage 1: facial clonus, including blinking, locomotor whiskers, rhythmic mastication, etc. Stage 2: including stage 1 and rhythmic nodding; Stage 3: fore clonus in addition to stage 2; Stage 4: including stage 3 and standing with hind legs; Stage 5: fall or jump, repeated convulsions or convulsions resulting in death on a stage 4 basis.

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Han Q.T., Yang W.Q., Zang C., Zhou L., Zhang C.J., Bao X., Cai J., Li F., Shi Q., Wang X.L., Qu J., Zhang D, & Yu S.S. (2023). The toxic natural product tutin causes epileptic seizures in mice by activating calcineurin. Signal Transduction and Targeted Therapy, 8, 101.

Publication 2023

Convulsions Epileptic Facial Legs Mastication Mice Tutin Whiskers

Corresponding Organization :

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Tutin

dependent variables

Epileptic activity of mice
Racine scale score (Stage 0: no reaction; Stage 1: facial clonus, including blinking, locomotor whiskers, rhythmic mastication, etc.; Stage 2: including stage 1 and rhythmic nodding; Stage 3: fore clonus in addition to stage 2; Stage 4: including stage 3 and standing with hind legs; Stage 5: fall or jump, repeated convulsions or convulsions resulting in death on a stage 4 basis)

control variables

Not explicitly mentioned

controls

Not explicitly mentioned

Annotations

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