Histological Analysis of Neuronal Density

Staining of brain tissues with hematoxylin and eosin (H&E) was done according to our previous work [33 (link)]. The hippocampal CA3 and CA1 regions and cerebellum were examined under a light microscope. Hippocampal regions were examined for the density of pyramidal cells, while the cerebellum was examined for the density of Purkinje cells. The number of Purkinje cells (PC) was counted in five sections of the cerebellum at magnification of 400× (high power field) in five rats at least, and the mean was calculated [34 (link)].

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Hussein A.M., Mahmoud S.A., Elazab K.M., Abouelnaga A.F., Abass M., Mosa A.A., Hussein M.A, & Elsayed M.E. (2023). Possible Mechanisms of the Neuroprotective Actions of Date Palm Fruits Aqueous Extracts against Valproic Acid-Induced Autism in Rats. Current Issues in Molecular Biology, 45(2), 1627-1643.

Publication 2023

Brain Cerebellum Eosin Hematoxylin Microscope light Purkinje cells Pyramidal cells Rats Staining tissues

Corresponding Organization : Mansoura University

Other organizations : Zagazig University, Jazan University, Delta University for Science and Technology, Universität Ulm, Carl von Ossietzky Universität Oldenburg

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Variable analysis

independent variables

Staining of brain tissues with hematoxylin and eosin (H&E)

dependent variables

Density of pyramidal cells in the hippocampal CA3 and CA1 regions
Density of Purkinje cells in the cerebellum

control variables

Magnification of 400× (high power field) used for counting Purkinje cells in the cerebellum

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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