Detecting LSDV Antibodies via ELISA

Serum samples were tested for the presence of antibodies to LSDV using ID Screen Capripox Double Antigen Multi-species ELISA (IDVet, Grabels, France, https://www.innovative-diagnostics.com/produit/id-screen-capripox-double-antigen-multi-species/; accessed on 23 January 2023) [28 ] recommended by the OIE Guidelines for Diagnostic Tests and Vaccines for Terrestrial Animals and performed according to the manufacturer instructions. This ELISA is designed to detect antibodies against Capripoxviruses including LSDV, sheep pox virus, and goat pox virus. Samples are read at 450nm and considered positive when the S/P% of the sample ≥30%. The S/P% is calculated according to: (OD of the sample/OD of the positive control) × 100.

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Hakobyan V., Sargsyan K., Kharatyan S., Elbakyan H., Sargsyan V., Markosyan T., Vardanyan T., Badalyan M, & Achenbach J.E. (2023). The Serological Response in Cattle following Administration of a Heterologous Sheep Pox Virus Strain Vaccine for Protection from Lumpy Skin Disease; Current Situation in Armenia. Veterinary Sciences, 10(2), 102.

Publication 2023

ID Screen Capripox Double Antigen Multi-species ELISA

Animals Antibodies Antigen Antigen species Capripoxviruses Diagnostic tests Diagnostics Elisa Goat pox virus Serum Sheep pox virus Vaccines

Corresponding Organization : Center for Ecological Noosphere Studies

Other organizations : Armenian National Agrarian University, Battelle

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Variable analysis

independent variables

None specified

dependent variables

Presence of antibodies to LSDV

control variables

Serum samples tested using ID Screen Capripox Double Antigen Multi-species ELISA
ELISA performed according to manufacturer instructions
Samples read at 450nm
Samples considered positive when S/P% ≥ 30%
S/P% calculated as (OD of sample/OD of positive control) × 100

controls

Positive control
Negative control

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