Analyzing YABBY Gene Expression in Monomorphella dodecandrum

The expression patterns of YABBY genes were analyzed by using RNA-seq data from different tissues of M. dodecandrum. After standardizing the data, TBtools was used to draw the heatmap of MdYABBYs [31 (link)]. To further analyze the expression patterns of the YABBY gene family in M. dodecandrum, RT-qPCR was performed. The RT-qPCR primers were designed by Primer3Plus (https://www.primer3plus.com/, accessed on 17 November 2022); Supplementary Table S1). A Taq Pro Universal SYBR qPCR Master Mix kit (Vazyme, Nanjing, China) was used for the RT-qPCR analysis. The MdActin gene was used as an internal reference [32 (link)], and the biological repetition and technical repetition were performed three times. The expression level was calculated by the 2^−∆Ct method [1 (link),32 (link)].