Antibodies used for immunofluorescence include anti-pH3 (Abcam, ab14955; 1:1,000), anti-Tbr1 (Abcam, ab31940; 1:300), anti-mCherry (Abcam, ab167453; 1:1,000), anti-NeuN (Millipore, MAB377; 1:300), anti-HA (Sigma-Aldrich, H6908; 1:2,000), anti-FLAG (Abcam, ab1162; 1:2,000), anti-LIC1 (Tan et al., 2011 (link); 1:300), and anti-LIC2 (Tynan et al., 2000a (link); 1:300). Donkey fluorophore–conjugated secondary antibodies (Jackson Labs, 1:500 dilution) were used.
Antibodies used for Western blotting include anti-p150Glued (BD, 610474; 1:1,000), anti–dynein IC clone 74.1 (University of Virginia, Charlottesville, VA; 1:1,000), anti-LICs (Tynan et al., 2000a (link); 1:500), anti-LIC1 (Tan et al., 2011 (link); 1:500), anti-LIC2 (Tynan et al., 2000a (link); 1:1,000), anti–dynein HC (Suzuki et al., 2007 (link); 1:1,000), and anti-GAPDH (Abcam, ab8245; 1:1,000). To develop in a LI-COR system, fluorescent secondary antibodies (1:10,000) were acquired from Invitrogen and Rockland.
Antibodies used for Western blotting include anti-p150Glued (BD, 610474; 1:1,000), anti–dynein IC clone 74.1 (University of Virginia, Charlottesville, VA; 1:1,000), anti-LICs (Tynan et al., 2000a (link); 1:500), anti-LIC1 (Tan et al., 2011 (link); 1:500), anti-LIC2 (Tynan et al., 2000a (link); 1:1,000), anti–dynein HC (Suzuki et al., 2007 (link); 1:1,000), and anti-GAPDH (Abcam, ab8245; 1:1,000). To develop in a LI-COR system, fluorescent secondary antibodies (1:10,000) were acquired from Invitrogen and Rockland.
