Cell lengths and widths of strains growing in BHI broth were measured from phase-contrast images by using Nikon NIS-Element AR software as described before (Barendt et al., 2009 (link), Tsui et al., 2014 (link)). For strains that do not show chaining morphology, separated stage 1 cells or daughter cells of stage 4 cells were measured. For strains that form short chains of cells, measurements included cells in chains whose widths at constriction sites were < 50% of cell widths. Fifty or more cells from two independent experiments were measured for each strain. P values were obtained by one-way ANOVA analysis (GraphPad Prism, nonparametric Kruskal-Wallis test).
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Cell Morphology Measurements in BHI Broth
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Corresponding Organization : Indiana University Bloomington
Other organizations : Molina Center for Energy and the Environment, Harvard University
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Variable analysis
independent variables
- Strain type (whether showing chaining morphology or not)
- Cell lengths
- Cell widths
- Growth media (BHI broth)
- Measurement method (Nikon NIS-Element AR software)
- Number of cells measured (50 or more from two independent experiments)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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