MicroRNA-381-3p Regulates Neuroinflammation

The pcDNA empty vector (NC, 5 μg/ml), pcDNA-CCR2 (CCR2, 5 μg/ml), the miRNA control (miR-NC, 50 nM), miR-381-3p mimics (miR-381-3p, 50 nM), the miRNA inhibitor (miR-in, 100 nM), and the miR-381-3p inhibitor (miR-381-3p-in, 100 nM) were supplied by GenePharma Co., Ltd. (Shanghai, China). OGD-elicited BV2 microglia and HT22 hippocampal neurons were seeded into 24-well cell culture plates with a density of 3 × 10⁵ cells/well. Transfection was conducted after they were cultivated in an environment of 37°C and 5% CO₂ for 24 hours. The above-mentioned RNAs were transfected into OGD-induced BV2 microglia and HT22 neurons employing Lipofectamine® 3000 (Invitrogen; ThermoFisherScientific, Inc.). qRT-PCR was carried out to determine the efficiency of the transfection [20 (link)]. The cells were incubated under the conditions of 37°C and 5% CO₂ for 24 hours in preparation for further analysis.