Detecting Antigen-Specific CD8+ T Cells

The following antibodies were purchased from BD Biosciences: anti–mouse CD8-APC (Ly-2), anti–mouse CD44-FITC (Pgp-1, Ly-24), and B220-Cy (RA3–6B2). CD40 (1C10 or FGK45) were produced by hybridomas that were grown in serum-free conditions. Ova-specific CD8 T cells were detected by H-2K^b–specific tetramers containing the SIINFEKL peptide, either made as described previously (33 (link)) (a gift from Dr. Lefrancois, University of Connecticut Health Center, Farmington, CT) or purchased from Beckman Coulter.

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Ahonen C.L., Doxsee C.L., McGurran S.M., Riter T.R., Wade W.F., Barth R.J., Vasilakos J.P., Noelle R.J, & Kedl R.M. (2004). Combined TLR and CD40 Triggering Induces Potent CD8+ T Cell Expansion with Variable Dependence on Type I IFN. The Journal of Experimental Medicine, 199(6), 775-784.

Publication 2004

Antibodies Cd8 t cells Fgk45 Fitc Hybridomas Mouse Pgp 1 Serum Siinfekl peptide Tetramers

Corresponding Organization :

Other organizations : Dartmouth College, 3M (United States), Bipar

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Protocol cited in 9 other protocols

Variable analysis

independent variables

Anti–mouse CD8-APC (Ly-2)
Anti–mouse CD44-FITC (Pgp-1, Ly-24)
B220-Cy (RA3–6B2)
CD40 (1C10 or FGK45)

dependent variables

Ova-specific CD8 T cells detected by H-2Kb–specific tetramers containing the SIINFEKL peptide

control variables

CD40 (1C10 or FGK45) produced by hybridomas grown in serum-free conditions

positive controls

H-2Kb–specific tetramers containing the SIINFEKL peptide made as described previously (33)

negative controls

None specified

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