The optimal formulation of HES plasma expander and the value of alternatives such as succinylated gelatin and methyl cellulose.
The use of low-speed centrifugation instead of sedimentation to separate LRP from red blood cells.
The added value of an optional additional centrifugation step to reduce the amount of contaminating platelets before labelling.
The use of PBS or saline as an alternative to CFP as the medium for cell labelling and resuspension.
The use of PRP instead of CFP, PBS or saline for centrifugation after labelling.
The use of PBS as an alternative to CFP for reconstitution of labelled WBC.
The need for gradient centrifugation and the availability of GMP grade gradient solutions for granulocyte purification.
Qualification and training required for personnel who perform WBC labelling.
Requirements for equipment and infrastructure for WBC labelling.
