Soluble IL-15/IL-15Rα complexes were prepared as described previously (9 (link), 35 (link)). A total of 2.5 mg rIL-15 (PeproTech) was mixed with 15 mg IL-15Rα (R&D Systems) in 50 μl PBS and incubated for 30 min at 37°C and then made up to a total volume of 300 μl with PBS prior to i.p. injection into WT and Ccr7−/− mice. Mice were sacrificed 4 d postinjection, and thymic samples were analyzed for iNKT cell populations by flow cytometry.
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