Fungal Pathogens and Streptococcal Serotypes

Aspergillus fumigatus (strain 13073) and isolates of Aspergillus niger (strain IMI 31274) and Aspergillus flavus (strain CBC B5107), all purchased from ATCC, were cultured on potato dextrose agar (Fisher) plates at 37°C for 5 days then harvested as described (16 (link)) in PBS, counted on a hemocytometer, and stored at 4°C. Group B streptococci, (strain names in parenthesis), GBS–Ib (H36B), GBSIa, GBSII (18RS21), and GBSIII (COH1) used in flow cytometry experiments and vaccinations were gifts from Dr. David Pritchard (UAB). GBS used for vaccines were grown to log phase, washed 3 times, and fixed overnight in 1% PFA at 4°C. mAbs specific for these GBS serotypes, α-1,3 glucan, and the anti-idiotype antibody, SMBi26 against SMB19 are described, together with methods of purification and quality control, in Supplementary Table 1.