Isolation and Analysis of Side Population Cells from Huh7 Hepatocellular Carcinoma
Side population (SP) and non-SP cells were analysed and sorted from Huh7 cells as described before [14 (link)] by using Hoechst33342 staining and MoFlo sorter (DAKOCytomation). Expression of the enzyme aldehyde dehydrogenase (ALDH) was analysed by using the ALDEFLUOR Kit from Stemcell Technologies (Grenoble, France) according to manufacturer's protocol after 24 hrs exposure of Huh7 cells to MTBITC. Cell sorting was performed with a BD FACS Aria III Cell Sorter (San Jose, CA, USA).
Herz C., Hertrampf A., Zimmermann S., Stetter N., Wagner M., Kleinhans C., Erlacher M., Schüler J., Platz S., Rohn S., Mersch-Sundermann V, & Lamy E. (2014). The isothiocyanate erucin abrogates telomerase in hepatocellular carcinoma cells in vitro and in an orthotopic xenograft tumour model of HCC. Journal of Cellular and Molecular Medicine, 18(12), 2393-2403.
Aldehyde dehydrogenase Aria Cell EnzymeHoechst33342 Side population cells Stemcell
Corresponding Organization :
Other organizations :
University Medical Center Freiburg, University of Freiburg, University of Hohenheim, University Medical Center of the Johannes Gutenberg University Mainz, Johannes Gutenberg University Mainz, University of Stuttgart, Universität Hamburg
Expression of the enzyme aldehyde dehydrogenase (ALDH)
Side population (SP) and non-SP cells
control variables
Hoechst33342 staining
MoFlo sorter (DAKOCytomation)
ALDEFLUOR Kit from Stemcell Technologies (Grenoble, France)
BD FACS Aria III Cell Sorter (San Jose, CA, USA)
controls
No positive or negative controls were explicitly mentioned in the input.
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