In this study, 60 adult Wistar albino rats (average weight: female rat 250-300 g, male rat 450-500 g) were randomly divided into 5 equal groups [6 males, 6 females (n = 12)]: group I (sham), group II (DOX), group III [treatment group I (DOX + P.v.L. hull extract 50 mg/kg)], group IV [treatment group II (DOX + P.v.L. hull extract 100 mg/kg)], group V (P.v.L. hull extract 100 mg/kg). The reason why we use both genders in rats, both male and female, is: heart diseases are found in people of both sexes, and one of the risk factors is gender. However, we could not obtain a statistically significant result in the evaluation made between rats according to their gender, so we presented the data in the general table without gender discrimination. Previously, male and female rats were treated with P.v.L. The response to this question was evaluated and compared separately. In order for the rats to adapt to the changing environmental conditions, they were housed for 5 days under routine housing conditions (temperature 22 ± 2ºC, 50% relative humidity, 12 hours of light and 12 hours of darkness, in type 3 cages with a transparent visible interior, designed to add standard rat chow, and ad libitum water) without any experimental intervention. All rats were fed with tap water and standard rat chow under standard conditions. The feeding of the rats was completely stopped 12 hours before the intervention. Female rats included in the study were placed in separate cages from male rats after the completion of the lactation period. During the study, experimental protocols were applied to rats grouped as male and female in separate cages. Due to this, vaginal plaque was not observed in female animals, and vaginal smear was not taken. Cardiac injury models were established as in previous study.18 For the experimental cardiac injury model, only food and water were given to group I for 15 days. In group II, DOX (2.5 mg/kg/day (15 days), a total dose of 37.5 mg/kg was administered intraperitoneally (IP). Group III was given only DOX for the first 7 days, then DOX and P.v.L. hull extract 50 mg/kg/day as gavage for 7 days. Group IV was given only DOX for the first 7 days, then DOX and P.v.L. hull extract 50 mg/kg/day as gavage for 7 days. In total, the duration of treatment was 15 days. Group V was given water for the first 7 days and P.v.L. hull extract by gavage for the next 7 days (Table 1). In total, the treatment lasted 15 days. At the end of the experiment (day 16), all rats were sacrificed under deep anesthesia (ketamine 90 mg/kg and xylazine 10 mg/kg IP), and their blood and all tissues were stored under appropriate conditions (Figure 1). Rats’ daily weight, feed, and water intake were followed up and noted. In the DOX-administered groups, the rats lost weight, their nutrition (feed and water consumption) decreased, they had diarrhea and nosebleeds and became weak. One male rat died in group II and group III.