Aorta-Caval Fistula Model in Mice

Mice used for this study included wild type female and male C57BL/6J, male Myh11-CreER^T2; Tgfbr2^fl/fl; mT/mG or male Cdh5-CreER^T2; Tgfbr1^fl/fl; Tgfbr2^fl/fl; mT/mG. C57BL/6J were purchased from The Jackson Laboratory (Bar Harbor, ME). Myh11-CreER^T2; Tgfbr2^fl/fl; mT/mG and Cdh5-CreER^T2; Tgfbr1^fl/fl; Tgfbr2^fl/fl; mT/mG were generated as previously described.^{22 (link),23 (link)} The bacterial artificial chromosome containing Myh11-CreER^T2 inserts on the Y chromosome and female mice do not express the construct. Based on prior studies, female mice have similar fistula dilation and wall thickening as male mice during days 0-21 in this aorta-caval fistula model despite different AVF inflow shear stress.^{24 (link)}

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Ryosuke T.(., Yuichi O.(., Jung S.L., Haidi H.(., Luis G., Weichang Z.(., John L., Yutaka M.(., Bogdan Y., George T., Tarek M.F., Katsuyuki H.(, & Alan D. (2022). Endothelial cell transforming growth factor-β signaling regulates venous adaptive remodeling to improve arteriovenous fistula patency. Arteriosclerosis, thrombosis, and vascular biology, 42(7), 868-883.

Publication 2022

C57BL/6J Myh11-CreERT2 Cdh5-CreERT2

Aorta Bacterial artificial chromosome Caval Cdh5 Dilation Female Fistula Male Mice Y chromosome

Corresponding Organization :

Other organizations : The University of Tokyo, Yale University, China Medical University, First Hospital of China Medical University, Kyushu University, VA Connecticut Healthcare System

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Variable analysis

independent variables

Mouse genotype: wild type female and male C57BL/6J, male Myh11-CreERᵀ²; Tgfbr2ᶠˡ/ᶠˡ; mT/mG, or male Cdh5-CreERᵀ²; Tgfbr1ᶠˡ/ᶠˡ; Tgfbr2ᶠˡ/ᶠˡ; mT/mG

dependent variables

Fistula dilation
Arterial wall thickening

control variables

Age and sex of mice (based on prior studies indicating similar responses in female and male mice)

positive controls

C57BL/6J wild-type mice

Annotations

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