Integrative Analysis of Glioma Transcriptome

The clinical and gene expression data of patients with LGGGBM were obtained from the TCGA database (https://cancergenome.nih.gov/) and the CGGA database (http://www.cgga.org.cn/). Normal brain RNA sequencing data were obtained from GTEx and utilized as normal control data. In this research, data from 609 glioma samples and 1152 normal brain samples were analyzed. Among them, the data used in the gene expression data were normalized. Next, DEGs were chosen using the R software’s “limma” package with the absolute value of the log2-transformed fold change (FC) > 1 and the adjusted p-value (adj. P) < 0.05 as the cutoff. In addition, the ComBat method was used to reduce batch effects with the R package “sva”.