Mouse brains were fixed in the 4% paraformaldehyde, and then fixed with paraffin. After that, 3 mm brain slices were immersed in 1% cresyl violet (50 °C, 1 h) and dehydrated with different ethanol solution, and then brain sections were cleared with xylene. Nissl-staining cells of cortex, hippocampal regions were imaged by light microscope (NIKON E600, Japan) and analyzed by Image-Pro Plus.
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