Isolation and Culture of hBMSCs

Human bone marrow mesenchymal stem cell were obtained according to procedures approved by the Ethics Committee at Maoming People's Hospital. hBMSCs were isolated from healthy volunteers and patients with OP. The hBMSCs were cultured under 10% exosome-free FBS for 72 h. The cells, dead cells and debris were removed using several low-speed centrifugations. hBMSCs of passage 5 were used for in our experiments. Cells were cultured in a humidified incubator with 5% CO₂ at 37 °C and passaged with trypsin/EDTA after reaching the confluence. The human osteosarcoma cell line, MG63 cell, was purchased from ATCC company. MG63 cells were cultured in a humidified incubator with 5% CO₂ at 37 °C with RPMI 1604 complete medium.