Infrared Thermography for Interscapular Brown Adipose Tissue

iBAT skin temperature was measured using infrared technology (camera FLIR E95 24°, Teledyne FLIR Systems, Wilsonville, OR, USA) and analyzed with its specific software, FLIR TOOLS Thermography Software. Videos were recorded on the first and the last day of treatment. As previously described, skin surrounding iBAT was shaved under isoflurane (IsoFlo^®, Zoetis, London, UK) anesthesia 2 days before recordings [27 (link)]. The increment of iBAT temperature was calculated as the difference of temperature from last day to first day of treatment.

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Fosch A., Rodriguez-Garcia M., Miralpeix C., Zagmutt S., Larrañaga M., Reguera A.C., Garcia-Chica J., Herrero L., Serra D., Casals N, & Rodriguez-Rodriguez R. (2023). Central Regulation of Brown Fat Thermogenesis in Response to Saturated or Unsaturated Long-Chain Fatty Acids. International Journal of Molecular Sciences, 24(2), 1697.

Publication 2023

IsoFlo

Anesthesia Isoflurane Skin Skin temperature Thermography

Corresponding Organization : Instituto de Salud Carlos III

Other organizations : Neurocentre Magendie, Inserm, Université de Bordeaux, Universitat de Barcelona

Top 5 similar protocols

Variable analysis

independent variables

Treatment duration

dependent variables

IBAT skin temperature

control variables

Isoflurane anesthesia
Shaving of skin surrounding iBAT 2 days before recordings

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