Genomic DNA extraction and Southern hybridization were performed as previously described [14 (link),17 (link)]. Briefly, mycelia were harvested using Miracloth filters and homogenized into powder in liquid nitrogen. Genomic DNA was isolated with phenol from mycelium grown shaking at 25°C in liquid PDM. Amersham AlkPhos Direct Labelling and CDP-Star detection reagents (GE Healthcare) were used for Southern hybridization.
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