Primary antibodies and their working dilutions used for western blotting included: mouse monoclonal anti-FLAG epitope (clone M2, Sigma) 1:5,000 and rabbit polyclonal anti-IgaA 1:10,000 [75 (link)]. Goat polyclonal anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Bio-Rad) were used as secondary antibodies at a 1:10,000 dilution. SDS-PAGE and western blotting were performed as described [76 (link)].
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