Quantifying Lignin Hydroxyl Groups
Corresponding Organization : University of Tennessee at Knoxville
Protocol cited in 2 other protocols
Variable analysis
- Lignin samples
- Hydroxyl groups of lignin samples measured using quantitative 31P NMR spectroscopy
- Amount of 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane (TMDP) used for derivatization (100 µL)
- Amount of derivatized samples (30 mg)
- Solvent composition (0.75 mL of pyridine and deuterated chloroform, 1.6:1 v/v)
- Concentration of N-hydroxy-5-norbornene-2,3-dicarboxylic acid imide (10 mg mL-1) and chromium(III) acetylacetonate (5 mg mL-1) used as internal standard and relaxation agent, respectively
- NMR parameters (inverse-gated decoupling pulse sequence, 90° pulse angle, 25 s relaxation delay, 256 scans)
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