Stem Cell Injection for Erectile Dysfunction

After sterilization and anesthesia by sodium pentobarbital (30 mg/kg, IP), the proximal shaft of the penis was tied gently by an elastic rubber band, and 0.2 ml of PBS was injected slowly into the middle of the corpus cavernosum (CC) of each rat in group II. Regarding group III, 2 × 10⁶ USCs labeled with a PKH-26 fluorescent cell linker (Sigma, St. Louis, MO, United States) were suspended with the same amount of PBS and injected into the CC of each rat in this group. Lastly, 200 μl of USC-L was injected into the CC of each animal in group IV. The needle and the rubber band were left in place after injection to prevent leakage (Woo et al., 2011 (link); Ouyang et al., 2014 (link)).

Free full text: Click here

Galhom R.A., Korayem H.E., Ibrahim M.A., Abd-Eltawab Tammam A., Khalifa M.M., Rashwan E.K, & Al Badawi M.H. (2022). Urine-Derived Stem Cells Versus Their Lysate in Ameliorating Erectile Dysfunction in a Rat Model of Type 2 Diabetes. Frontiers in Physiology, 13, 854949.

Publication 2022

PKH-26 fluorescent cell linker

Anesthesia Animal Cell Needle Penis Pkh 26 Rubber Sodium pentobarbital Sterilization

Corresponding Organization : Al Jouf University

Other organizations : Beni-Suef University, Cairo University, King Saud University, Al-Azhar University, Helwan University

Top 5 similar protocols

Variable analysis

independent variables

Injection of 0.2 ml of PBS into the middle of the corpus cavernosum (CC) of each rat in group II
Injection of 2 × 10^6 USCs labeled with a PKH-26 fluorescent cell linker suspended in the same amount of PBS into the CC of each rat in group III
Injection of 200 μl of USC-L into the CC of each animal in group IV

dependent variables

Not explicitly mentioned

control variables

Sterilization and anesthesia by sodium pentobarbital (30 mg/kg, IP)
Tying the proximal shaft of the penis gently by an elastic rubber band
Leaving the needle and the rubber band in place after injection to prevent leakage

controls

Group II received PBS injection as a control group
Group III received USC injection as the experimental group

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!