Western Blot Analysis of Cell Signaling

Western blots were done as previously described [37 (link)], with the primary antibodies: anti-AKT (11E7), anti-phospho-AKT(Ser⁴⁷³), anti-p65 NFκB subunit (E498), anti-phospho-p65 (Ser⁵³⁶), anti-IκBα (L35A5), anti-caspase 3,anti-caspase 9, anti-c-Myc and anti-phospho-GSK3αβ (Ser^21,9) from Cell Signaling Technology; anti-BCL2, anti-BAX (6a7), anti-survivin, anti-cyclin D1 and anti-β-actin (AC-15) from Sigma; anti-BCL-X_L and anti-PARP (C2-10) and anti-p53 from BD Biosciences; anti-phospho-histone H2A.X (Ser¹³⁹) and anti-cIAP (315301) from R&D Systems and anti-GSK3β from Santa Cruz Biotechnologies.

Free full text: Click here

Rebolleda N., Losada-Fernandez I., Perez-Chacon G., Castejon R., Rosado S., Morado M., Vallejo-Cremades M.T., Martinez A., Vargas-Nuñez J.A, & Perez-Aciego P. (2016). Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model. PLoS ONE, 11(4), e0154159.

Publication 2016

anti-caspase 9 anti-c-Myc anti-phospho-GSK3αβ (Ser21,9) anti-cyclin D1 anti-β-actin (AC-15) anti-p53 anti-GSK3β

Actin Antibodies Bcl2 Caspase 3 Caspase 9 Cyclin d1 Gsk3β Histone h2a x Iκbα Nfκb Subunit Survivin Western blots

Corresponding Organization : Hospital Universitario La Paz

Top 5 similar protocols

Variable analysis

independent variables

Western blotting protocol

dependent variables

Levels of AKT, phospho-AKT (Ser473), p65 NFκB subunit, phospho-p65 (Ser536), IκBα, caspase 3, caspase 9, c-Myc, phospho-GSK3αβ (Ser21,9), BCL2, BAX, survivin, cyclin D1, BCL-XL, PARP, phospho-histone H2A.X (Ser139), cIAP, and GSK3β

control variables

β-actin

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!