MCF7 and MDA-MB-330 (MM330) (American Type Culture Collection [ATCC], Manassas, VA, USA) were cultured in DMEM (11965; Life Technologies, Carlsbad, CA, USA) +10%FBS (26140; Life Technologies). T47D (ATCC) and ZR75.1 (ATCC) were cultured in RPMI 1640 (11875; Life Technologies) +10%FBS. MDA-MB-134VI (MM134) (ATCC) and SUM44PE (Asterand Bioscience, Detroit, MI, USA) were maintained as described previously78 (link). All lines were incubated at 37 °C in 5% CO2.
Cycloheximide (C4859; Sigma-Aldrich, St. Louis, MO, USA), 4EGI-1 (S7369; Selleck Chemicals, Houston, TX, USA), and Salubrinal (SC-202332A; Santa Cruz, Dallas, TX, USA) were dissolved in DMSO (4-X; ATCC).
Cycloheximide (C4859; Sigma-Aldrich, St. Louis, MO, USA), 4EGI-1 (S7369; Selleck Chemicals, Houston, TX, USA), and Salubrinal (SC-202332A; Santa Cruz, Dallas, TX, USA) were dissolved in DMSO (4-X; ATCC).
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