The GUS activity of the mice was detected using fluorescein di-β-D-glucuronide (FDGlcU, Thermo Fisher Scientific, Cat#F2915) by the method of Chen et al. as reported.38 (link) Mice (n = 6, per group) were administrated orally 7.3 μmol/kg FDGlcU dissolved in distilled deionized water. The fluorescence intensity in the intestine was detected on a Spectrum imaging system (Lago X, Spectral Instruments Imaging) 3 h after probe administration (λex: λem = 465 nm: 530 nm), with an exposure time was 5s. Fluorescence intensity was quantified by intestinal fluorescence using the Aura assay software.
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In-Vivo GUS Activity Assay
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Corresponding Organization : Huazhong University of Science and Technology
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Variable analysis
independent variables
- Dose of FDGlcU (7.3 μmol/kg) administered orally to mice
- Fluorescence intensity in the intestine detected using a Spectrum imaging system (Lago X, Spectral Instruments Imaging)
- Number of mice per group (n = 6)
- Exposure time for fluorescence detection (5s)
- Excitation and emission wavelengths (λex: λem = 465 nm: 530 nm)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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