Human small cell lung cancer cell lines (NCI-H417, NCI-H82, NCI-H2171, NCI-H847, NCI-H1048, NCI-H146, NCI-H510A, NCI-H1963, and NCI-H1876) were kindly provided by Dr. Adi Gazdar at the University of Texas Southwestern. Cells were cultured in RPMI (GE Lifesciences) supplemented with 10% heat-inactivated FBS. Cell lines were tested for and free of mycoplasma, and cell line identities were verified using short tandem repeat genotyping as compared with the original primary sample material within the CCcells database: www.CCcells.org . Small cell lung cancer cells (2 × 106 cells) were plated in 96-well plates for 24 h before treatment with inhibitors (1 nM–10 μM in 3× increment). Six replicates of each drug concentration were used. After 96 h of drug incubation, cellular viability was measured using the DIMSCAN assay, as outlined in previous studies (Kang et al., 2011 (link); Zhang et al., 2012 (link)).
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Small Cell Lung Cancer Cell Line Viability Assay
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Assay
Cell line
Cells
Cellular viability
Drug
Human
Inhibitors
Mycoplasma
Short tandem repeat
Small cell lung cancer
Corresponding Organization : Texas Tech University
Other organizations : Pennington Biomedical Research Center, The University of Texas at Austin
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Variable analysis
independent variables
- Concentration of inhibitors (1 nM–10 μM in 3× increment)
- Cellular viability (measured using the DIMSCAN assay)
- Cell lines (NCI-H417, NCI-H82, NCI-H2171, NCI-H847, NCI-H1048, NCI-H146, NCI-H510A, NCI-H1963, and NCI-H1876)
- Cell culture conditions (RPMI supplemented with 10% heat-inactivated FBS)
- Cell plating density (2 × 10^6 cells per 96-well plate)
- Cell line identity verification (using short tandem repeat genotyping and comparison with the original primary sample material within the CCcells database)
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