Sample preparation and experimental parameters have been previously described in detail for ESI studies, but a few details will be mentioned here.1 (link) Methylenedianiline (MDA) 2-ring isomers (2,2′-MDA, 2,4′-MDA, and 4,4′-MDA) were provided by Dr. Stefan Wershofen (Bayer MaterialScience AG, 47812 Krefeld, Germany). Methanol and water with 0.1% formic acid were obtained from Fisher Scientific (Waltham, MA). Tetralkylammonium salts, α-cyano-4-hydroxycinnamic acid (CHCA), alkali salts, and 2,7-diaminofluorene (2,7-DAF) were obtained from Sigma-Aldrich (St. Louis, MO). Samples were dissolved at a concentration of 1 mg/mL in 9:1 methanol/water containing 0.1% formic acid (v/v). Each MDA isomer was combined in a 10:1 matrix-to-analyte ratio and 2 to 3 layers of 0.5 μL of sample were spotted on a 100-well MALDI plate.
Protocol detail
MALDI-MS Analysis of MDA Isomers
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Virginia Innovation Partnership Corporation, Vanderbilt University
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Methylenedianiline (MDA) 2-ring isomers (2,2′-MDA, 2,4′-MDA, and 4,4′-MDA)
- Not explicitly mentioned
- Methanol and water with 0.1% formic acid
- Tetralkylammonium salts
- α-cyano-4-hydroxycinnamic acid (CHCA)
- Alkali salts
- 2,7-diaminofluorene (2,7-DAF)
- Concentration of samples (1 mg/mL in 9:1 methanol/water containing 0.1% formic acid (v/v))
- Matrix-to-analyte ratio (10:1)
- Spotting volume (2 to 3 layers of 0.5 μL of sample)
- No positive or negative controls were explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!