Cancer Stem Cell Modeling in Lung Cancer

A549 and H1299 human lung cancer cell lines were obtained from the Stem Cell Bank of the Chinese Academy of Sciences. Cells were cultured with Dulbecco’s modified Eagle medium (DMEM) supplemented with 1% amphotericin B, 1% penicillin–streptomycin, and 10% fetal bovine serum. A549 and H1299 CSC derivative cell lines, the A549-oncosphere, and the H1299-oncosphere were generated as previously described^{38 (link)}. Cells were cultured in the DMEM/F12 supplemented with 2% B27, 1% amphotericin B, and 1% penicillin–streptomycin, with 20 ng/ml epidermal growth factor (Beyotime) and 20 ng/ml fibroblast growth factor (Beyotime).