Quantifying Apoptosis in C. elegans

Adult nematodes were suspended in M9 solution and stained by incubation with 33 µM SYTO-12 (Molecular probes) for 1 h and 30 min at room temperature in the dark. The worms were then transferred to seeded plates to allow stained bacteria to be purged from the gut. After 30 min, the animals were mounted on 2% agarose pads in 2 mM levamisole^{21 (link)}. The estimation of apoptotic levels for each genotype was calculated as the average number of apoptotic nuclei per gonadal arm^{22 (link)}.

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Santonicola P., Germoglio M., d’Abbusco D.S, & Adamo A. (2020). Functional characterization of Caenorhabditis elegans cbs-2 gene during meiosis. Scientific Reports, 10, 20913.

Publication 2020

SYTO-12

Adult Agarose Animals Apoptotic Bacteria Genotype Gonadal Molecular probes Nematodes Nuclei Worms

Corresponding Organization :

Other organizations : Institute of Biosciences and Bioresources, National Research Council

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Variable analysis

independent variables

Adult nematodes were suspended in M9 solution
Adult nematodes were stained by incubation with 33 µM SYTO-12 (Molecular probes) for 1 h and 30 min at room temperature in the dark

dependent variables

Estimation of apoptotic levels for each genotype, calculated as the average number of apoptotic nuclei per gonadal arm

control variables

Adult nematodes were transferred to seeded plates to allow stained bacteria to be purged from the gut
Adult nematodes were mounted on 2% agarose pads in 2 mM levamisole

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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