FIV reporter assay was performed as previously described (22 (link), 24 (link), 36 (link), 62 (link)). Briefly, 10 μl of the culture supernatant of transfected cells was inoculated into HEK293T cells in a 96-well plate (Nunc). Firefly luciferase activity was measured by using the BrillianStar-LT assay system (Toyo-b-net) and the 2030 ARVO X multilabel counter instrument (PerkinElmer) according to the manufacturers’ procedures.
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