The expression plasmids for wt E1A12 and various mutants are reported previously (38 (link)). The full-length AR (GFP-AR, plasmid # 28235) and AR-V7 (GFP-AR-V7, plasmid # 86856) fused to GFP were obtained from Addgene. The mCherry-AR construct was made by inserting the full-length AR cDNA fragment from the GFP-AR plasmid at the 3’ end of the mCherry coding sequence. FLAG-tagged AR NTD (1–566) and AR NTD-DBD-Hinge (1–802) were generated by PCR using the GFP-AR as template and cloned into pExchange-3B. FLAG-tagged full-length AR and constructs with a specific deletion within the AR NTD were described (39 (link)). Ad-E1A12 and other viruses with mutated E1A12 were constructed as reported previously (38 (link)). Recombinant viruses were generated through homologous recombination between pShuttle-CMV carrying the E1A12 expression cassette and pAdEasy-1, an E1 and E3-deleted Ad vector (Agilent Technologies) (40 (link)). Ad-E1A12 was packaged in HEK293 cells. The recombinant viruses expressing a mutated E1A12 were constructed similarly. A large-scale preparation of purified Ad-E1A12 viral particles was done by ViraQuest, Inc. Ad-eGFP and wild-type (wt) Ad5 were purchased from ViraQuest, Inc.