SNP Genotyping for Genetic Profiling

Genomic DNA has been extracted previously from blood samples with the QIAamp DNA Blood Midi kit (Qiagen, Venlo, The Netherlands), and from 122 fresh-frozen primary tumour samples with the High Pure PCR Template Preparation Kit (Roche, Vienna, Austria) as described [27] (link), [28] (link). Genotyping of SNP rs1800449 (G473A; c.473G>A; Arg158Gln; R158Q) in the LOX gene was performed by TaqMan PCR with Genotyping Mastermix and allele-specific, fluorescently labeled probes on a 7500 fast instrument following the manufacturer’s instructions (Applied Biosystems, Brunn/Gebirge, Austria; Assay-ID #C___7574719_10). 20 ng of genomic DNA were used per reaction in a reaction volume of 10 µl.