Oxidative Stress Biomarkers Quantification

Plasmatic lipid peroxidation as a consequence of oxidative stress was estimated by measuring thiobarbituric acid reactive substances (TBARS) using a kit (OxiSelect™ TBARS Assay Kit-MDA Quantitation, Cell Biolabs Inc., San Diego, CA, USA) according to the manufacturer’s instructions, and expressed in µmol/L malondialdehyde (MDA). Superoxide dismutase (SOD) and catalase activities were measured according to the manufacturer’s instructions (Superoxide dismutase assay kit and Catalase Assay Kit, Abcam, Paris, France) and expressed, respectively, in percent of inhibition rate and μmol/L. Total antioxidant capacity (TAOC) with the radical cation ABTS^•+ (2,2′-azino-bis-(3)-ethylbenzthioazoline-6-sulfonic acid, VWR, Fontenay sous Bois, France) was determined using 6-hydroxy-2, 5, 7, 8-tetramethylchromane-2-carboxylic acid (Trolox; Sigma-Aldrich, St Quentin Fallavier, France) equivalent, as previously described for plasma [1 (link)].