Antibody Sources and Reagents for Protein Analysis

The primary antibody against SNX9 (OTI1E4) was purchased from Origene, anti-EGFR (PA1-1110) and anti-GFP (MA5-15256) were from Thermo Scientific, antipenta-His (#34660) from Qiagen, anti-GST (#2622) from Cell Signaling, and anti-DsRed (sc-101526) from Santa Cruz. Anti-IncA was a gift from G. Zhong, University of Texas Health Science Center at San Antonio, San Antonio, TX (57 (link)), and anti-DnaK was obtained from S. Birkelund, Aalborg University, Aalborg, Denmark (58 (link)). Antibodies against Cpn0677 and Cpn0678 were generated in our laboratory, as was anti-CPn0147. Mouse anti-Cya, rabbit anti-CRP, and rabbit anti-IpaD antibodies were generously donated by N. Guiso, A. Ullmann, and C. Parsot, Institut Pasteur, Paris, France, respectively. Secondary anti-rabbit and anti-mouse antibodies coupled to Alexa488 or Alexa594 were purchased from Thermo Scientific, and those coupled to alkaline phosphatase were sourced from Promega. CellMask (orange) and Rhodamine-Phalloidin were purchased from Thermo Scientific, and SiR-Tubulin from Spirochrome. All lipids used in this study were obtained from Avanti Lipids, and Texas red dye, NHS-FITC and DyLight650NHS from Thermo Scientific. Nocodazole and Cytochalasin D were purchased from Merck.

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Hänsch S., Spona D., Murra G., Köhrer K., Subtil A., Furtado A.R., Lichtenthaler S.F., Dislich B., Mölleken K, & Hegemann J.H. (2020). Chlamydia-induced curvature of the host-cell plasma membrane is required for infection. Proceedings of the National Academy of Sciences of the United States of America, 117(5), 2634-2644.

Publication 2020

OTI1E4) MA5-15256) antipenta-His anti-GST alkaline phosphatase CellMask (orange) Rhodamine-Phalloidin SiR-Tubulin Texas red dye NHS-FITC Nocodazole Cytochalasin D

Alexa594 Alkaline phosphatase Anti antibodies Antibodies Antibody Cytochalasin d Egfr Fitc Lipids Mouse Nocodazole Promega Rabbit Rhodamine phalloidin Tubulin

Corresponding Organization :

Other organizations : Heinrich Heine University Düsseldorf, Institut Pasteur, Centre National de la Recherche Scientifique, German Center for Neurodegenerative Diseases, University of Bern

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Variable analysis

independent variables

Primary antibodies against SNX9 (OTI1E4), EGFR (PA1-1110), GFP (MA5-15256), penta-His (#34660), GST (#2622), DsRed (sc-101526), IncA, DnaK, Cpn0677, Cpn0678, CPn0147, Cya, CRP, IpaD

dependent variables

Not explicitly mentioned

control variables

Secondary anti-rabbit and anti-mouse antibodies coupled to Alexa488 or Alexa594, and those coupled to alkaline phosphatase
CellMask (orange), Rhodamine-Phalloidin, SiR-Tubulin
All lipids used in this study, Texas red dye, NHS-FITC, DyLight650NHS
Nocodazole, Cytochalasin D

positive controls

Anti-IncA: gift from G. Zhong, University of Texas Health Science Center at San Antonio
Anti-DnaK: obtained from S. Birkelund, Aalborg University

negative controls

Not explicitly mentioned

Annotations

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