The human chondrocyte cell line CHON-001 and 293T cells were obtained from the American Type Culture Collection. Cells were cultured in RPMI-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Invitrogen; Thermo Fisher Scientific, Inc.) and 2 mM glutamine (Sigma-Aldrich; Merck KGaA) andplaced at 37˚C in a humidified incubator containing 5% CO2. CHON-001 cells were treated with 10 ng/ml interleukin (IL)-1β (Sigma-Aldrich; Merck KGaA) at room temperaturefor 24 h to generate an in vitro model of OA (16 (link)). The nuclear factor κB (NF-κB) inhibitor BAY 11-7085 (10 µM) was purchased from MedChemExpress.
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Chondrocyte Model of Osteoarthritis
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293t cells
Bay 11 7085
Cell line
Cells
Chondrocyte
Cultured medium
Fetal bovine serum
Glutamine
Human
Interleukin 10
Interleukin 1β
Nuclear factor κb inhibitor
Corresponding Organization :
Other organizations : Second Affiliated Hospital of Inner Mongolia Medical University
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Variable analysis
independent variables
- Interleukin (IL)-1β (10 ng/ml)
- NF-κB inhibitor BAY 11-7085 (10 µM)
- Cellular response to IL-1β treatment
- Effect of NF-κB inhibitor on cellular response
- CHON-001 cell line
- 293T cell line
- RPMI-1640 medium supplemented with 10% fetal bovine serum and 2 mM glutamine
- Incubation at 37°C in a humidified 5% CO2 incubator
- Positive control: CHON-001 cells treated with IL-1β (10 ng/ml) to generate an in vitro model of osteoarthritis (OA)
- Negative control: Not explicitly mentioned
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