Ookinete microneme secretion was analyzed as previously reported (47 (link)). Briefly, about 2.0 × 106 of ookinetes were purified from in vitro culture using the LS magnetic column (#130-042-401, Miltenyi Biotec) and incubated in 500 μl of PBS for 4 hours at 22°C. The cells were spun down at 750g, and the supernatant was collected, filtered through a filter (0.45 μm; SLHP033RS, Millipore), and subjected to Western blot assay.
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Ookinete Microneme Secretion Protocol
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Other organizations : Xiamen University
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Variable analysis
independent variables
- Incubation time (4 hours)
- Ookinete microneme secretion
- Number of ookinetes (2.0 × 10^6)
- Incubation temperature (22°C)
- Incubation medium (PBS)
- Positive control: Not mentioned
- Negative control: Not mentioned
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