BrdU labeling and immunostaining were used to evaluate cell proliferation in mouse lung sections [23 (link), 25 (link)]. BrdU (75mg/kg BW) was administered i.p. to mice at 4h prior to tissue collection. Mouse lung cryosections (5 μm) were stained with FITC-conjugated anti-BrdU using the In Situ Cell Proliferation kit following the manufacturer’s instructions (Roche Applied Science). Endothelial cells were stained with anti-CD31 (1:40, Abcam) and -vWF (1:250, Sigma-Aldrich) antibodies while nuclei were counterstained with DAPI (Invitrogen).
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Evaluating Cell Proliferation in Murine Lungs
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Corresponding Organization : University of Illinois Urbana-Champaign
Other organizations : Jiangsu University
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Variable analysis
independent variables
- BrdU (75mg/kg BW) administered i.p. to mice at 4h prior to tissue collection
- Cell proliferation in mouse lung sections
- Mouse lung cryosections (5 μm)
- Staining with FITC-conjugated anti-BrdU using the In Situ Cell Proliferation kit
- Staining of endothelial cells with anti-CD31 (1:40, Abcam) and -vWF (1:250, Sigma-Aldrich) antibodies
- Nuclei counterstained with DAPI (Invitrogen)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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