Snai2 Knockdown and Overexpression

Embryos were injected at the one-cell stage with morpholino oligonucleotides (MOs, GeneTools) and/or mRNA. Antisense MOs were used at the following concentrations: 10 ng snai2 splice-block MO (SB MO)[15 (link),44 (link)] and 0.75 ng snai2 5’UTR MO (UTR MO). Capped mRNA was synthesized from linearized pCS2⁺ constructs using the mMessage mMachine SP6 Transcription Kit (ambion, AM1340), according to manufacturer’s recommendations. Full length snai2 mRNA was injected into embryos at 150 ng/μl.

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Bickers C., Española S.D., Grainger S., Pouget C, & Traver D. (2018). Zebrafish snai2 mutants fail to phenocopy morphant phenotypes. PLoS ONE, 13(9), e0202747.

Publication 2018

morpholino oligonucleotides (MOs, mMessage mMachine SP6 Transcription Kit

Cell Embryos Morpholino oligonucleotides Mrna Transcription

Corresponding Organization : University of California, San Diego

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Variable analysis

independent variables

Morpholino oligonucleotides (MOs) injected at the one-cell stage
MRNA injected at the one-cell stage

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Full length snai2 mRNA injected into embryos at 150 ng/μl

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