Quantification of Artificial Sweeteners

Standards of artificial sweeteners and steviol glycosides were obtained from different sources: acesulfame-K from Nutrinova (Frankfurt am Main, Germany); saccharine, sucralose and neohesperidin DC from Sigma-Aldrich (St. Louis, USA); aspartame from Ajinomoto Foods Europe (Nesle, France); cyclamate from Merck KGaA (Darmstadt, Germany); alitame from Frapp’s Pharma (Hong Kong, China); neotame from CHEMOS (Regenstauf, Germany); and rebaudioside A, stevioside, rebaudioside C, dulcoside A, steviolbioside and steviol from LGC Standards (Łomianki, Poland). As the internal standard (IS), sodium N-(2-methylcyclohexyl)sulfamate was used [27 (link)]. Acetonitrile (ACN), methanol (MeOH) and acetone were purchased from Merck KGaA (Darmstadt, Germany). Acetic acid (AA) was obtained from POCH (Gliwice, Poland). Ultrapure water was prepared using the HLP5 system from Hydrolab (Wiślina, Poland).